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Abstrak

Centella asiatica DNA Barcoding: Assessing Antibacterial Potential and Environmental Factors


Oleh :
Lamlom Taie Lamlom Tahawy - S902208003 - Fak. MIPA

Centella asiatica, often known as Gotu Kola, has a rich and extensive history in the field of traditional medicine. Recent experiments have shown that these plants have the potential to be reservoirs of antibacterial compounds, specifically those targeting MRSA. The antibacterial properties of C. asiatica from various regions are still not adequately characterized. This thesis examines the genetic diversity, bioactive component profile, and antibacterial activity of C. asiatica populations in Tawangmangu, Central Java, Indonesia. The study aims to identify populations that fight MRSA and review the impact of environmental variables on genetic diversity and plant bioactivity. Methods: DNA barcodes with the rbcL gene were used to study genetic diversity in samples from Kemuning, Sekipan, and Taman Hutan Rakyat. Antimicrobial activity against MRSA was evaluated using
disc diffusion. The effects of pH, light intensity, humidity, and temperature on the synthesis of bioactive chemicals were studied. Results: DNA barcoding revealed significant genetic diversity, with Kemuning showing distinct genetic lineages and People's Forest Park showing divergence. The environment significantly influences the synthesis of saponins and terpenoids, with the People's Forest Park showing the highest amounts. However, no extract from C. asiatica was effective in reducing MRSA at the amounts studied. Antimicrobial potency test: Lack of antimicrobial agent amounts, inadequate extraction methods, orgenetic variations affecting the synthesis of these compounds may explain the lack  of antibacterial effectiveness against MRSA, despite the presence of saponins and terpenoids. The study of medicinal plants such as C. asiatica requires a deep understanding of genetic diversity and environmental conditions. The next research goal should be to improve extraction methods, learn more about the genetics of the synthesis of bioactive components, and discover the environmental factors that help C. asiatica make more antibacterial substances