PENGARUH ASPARTAM TERHADAP KADAR KREATININ SERUM DAN STRUKTUR HISTOLOGIS REN MENCIT (Mus musculus L.) STRAIN SWISS

Oleh :
Umi Barokah Irawati - M0402047 -

ABSTRACT The aims of this research were to know the effect of aspartame to the serum creatinine level and histologic structure of kidney (glomerules and proximal tubules). This research was evaluated experimentally to male mice with average weight of 20 gr. These mice were divided randomly in to 5 groups i.e.: control, 5 mg aspartame, 10 mg aspartame, 15 mg aspartame, and 20 mg aspartame. The treatment was done for 28 days, before treatment blood had taken and measured creatinine serum level. After treatment mice blood had taken again, killed and sectioned kidney organ to make histologic preparation. Creatinine serum level, wide average Bowman space glomeruli and lumen proximal tubules was analyzed using analysis of varians (ANOVA) and continued Duncan Multiple Range Test (DMRT) on 5% significans, time factor of creatinine serum level was analyzed using T-test. Observation of kidney histologic structure (glomerules and proximal tubules) were done to determine the kind and level of damage and analyzed descriptively. Creatinine serum level control normaly, dosage variation aspartame creatinine serum level increased up to ± 3.29 mg/dl. There were cantrol glomerules and proximal tubules normaly. Dosage variation aspartame glomerules damage, i.e.: cloudy swelling degeneration; karyorectic; picnotic; karyolitic; wide Bowman space and hemolitic erytrocyte, proximal tubules damages were picnotic; karyorectic; karyolitic; hydrophic degeneration; lumen narrowed and protein cast. Key Word : aspartame, creatinine, glomerules, proximal tubules