UJI SITOTOKSISITAS CAMPURAN EKSTRAK Pandanus conoideus Lamb. VARIETAS BUAH KUNING DAN ASAM LAURAT DARI VCO TERHADAP SEL KANKER PAYUDARA T47D SECARA IN VITRO

Oleh :
Auronita Puspa Pratiwi - M0405018 -

ABSTRACT Investigation on cell line T47D using extract Pandanus conoideus red variety showed the extract could reactivate p53 mutation. This effect was expected caused by interaction of
-tocopherol dan -carotene which it’s contain (Astirin and Budiani, 2007). Pandanus conoideus yellow variety has known containing tocopherol higher than red variety (Budi and Paimin, 2005). Other investigation showed LC50 P.conoideus yellow variety extract was at concentration 0,25µl/ml (Pratiwi, unpublished). Wibowo et al (2007), showed that lauric acid from VCO has cytotoxic activity on cell line T47D, MCF7, HT29, Raji, and HeLa. The aim of this investigation has examined potentiation effect of cytotoxicity and antiproliferative activity of combination of P.conoideus yellow variety extract and lauric acid from VCO, and found effective concentration in proliferative inhibition on breast cancer cell line T47D This investigation included cytotoxicity and doubling time tested by direct counting method. In cytotoxicity test, the cells had incubated for 24hr in RPMI 1640 media after added combination of yellow variety extract and lauric acid from VCO in six group i. e control group and six concentration of lauric acid (0,1µl/ml, 0,05µl/ml, 0,025µl/ml, 0,0125µl/ml and 0,00625µl/ml) combined 0,25µl/ml P.conoideus. Proliferatif inhibition activity has known by doubling time test for 72hr. Cell amount counted at 0, 24, 48, and 72hr. Investigation resulted LC50 of combination of P.conoideus yellow variety extract and lauric acid from VCO was in 0,25µl/ml P.conoideus and 0,0125µl/ml lauric acid. Effective concentration on doubling time test was found in 0,125µl/ml P.conoideus and 0,00625µl/ml lauric acid. The investigation also showed hat the combination has sinergistic effect in cytotoxic and inhibition than using P.conoideus yellow variety extract and lauric acid alone. Keywords: P.conoideus yellow variety, Lauric acid, cytotoxicity, cell line T47D