EFEK TOKSISITAS KOMPONEN BIOAKTIF DAUN LOBAK (Raphanus sativus Landra. var. hortensis Back.) DENGAN METODE BRINE SHRIMP LETHALITY TEST SEBAGAI KANDIDAT ANTIKANKER DAN PROFIL KROMATOGRAFI LAPIS TIPISNYA

Oleh :
Yustin Nur Khoiriyah - M0405065 -

ABSTRACT Cancer have a great rank position to cause of people death in developing country (Indrayani et al., 2006). The synthetic drug of cancer to healing trade have been give side effects and almost expensive relatively. This reason is push ahead to discover a new source drug of cancer. Indonesia have a great potency in this case. One of the plant which have potency to be a new source drug of anticancer candidate is Lobak (Raphanus sativus L.). This vegetables as drug usefull have various bioactive compound on this leaves and tuber, and it has been used empirical for anticancer drugs. The new source drug as a candidate of anticancer require to be proved scientifically. In this research was conducted toxicity effect from bioactive compound of Lobak (Raphanus sativus L.) leaves with BST method as pre screening to discover an anticancer compound. The brine shrimp lethality test as toxicity test has been done by including ten larva A. salina Leach into flacon contain test sampel. Death percentage of A. salina Leach larva was counted 24 hours after giving of test sampel rate series, then made equation of linear line to determine values of LC50-24 hours. The result of toxicity test showed that methanol extract more toxic than chloroform extract. The partition result of methanol extract showed that insoluble fraction of ethyl acetate more toxic than soluble fraction of ethyl acetate. Insoluble fraction of acetate ethyl as toxic fraction, then be partitionated with acetonitril. The test result of partition with acetonitril by BST showed that insoluble fraction acetonitril is more toxic than soluble fraction of acetonitril with values of LC50-24 hours = 90,54
g/mL. TLC chromatogram with various specific reagent showed that insoluble fraction of acetonitril found a phenolic compound. Keyword : Raphanus sativus L. var hortensis Back, toxicity, BST, and anticancer