UJI ANTIBAKTERI KOMPONEN BIOAKTIF DAUN LOBAK (Raphanus sativus L.) TERHADAP Escherichia coli DAN PROFIL KANDUNGAN KIMIANYA

Oleh :
Jenny Virganita - M0405033 -

ABSTRACT Indonesia has many kind of plants, which some of them are medicinal plants, and it have been used to cure various disease. One of medicinal plants is Radish (Raphanus sativus L.), known as vegetable. That’s the reason conducting some research to find the bioactive compound from plants, which can used as raw material of drug. Another reasons are costly drug prices in market, the death case due to microbial infections, and the increasing of bacterial resistance because of uneffectiveness usage of antibiotics. This study was aimed to know the antibacterial effect of bioactive compounds from Radish’s leaves against Escherichia coli. Bioactive compounds were found by extraction dan partition using sentrifugation. Dry powder from Lobak leaves was extracted by chloroform and methanol. After the antibacterial tested, methanol extract given an antibacterial effect so it had separated in partition by ethyl acetate into the soluble and insoluble part. The soluble part of ethyl acetate given an antibacterial effect on concentration 30-50%, shown by clear zone (average 10 mm). It had separated again into soluble and insoluble fraction with dicloromethane. Dicloromethane’s soluble fraction formed clear zone on 10% concentration (8,30 mm) and 8,42 mm on 20%. Then the chemical constituen’s profile of the most active fraction has been monitored using KLT method and detected with spray chemicals. The result indicating that Radish’s leaves have bioactive compound from fenolic group. It has been proved by ammonia detection which was gave a yellow colored, and dark blue with FeCl3 detection. Keywords: Raphanus sativus L, Escherichia coli, antibacterial test, bioactive compounds