Banana propagation generally uses suckers or offshoots, which only produce limited seedlings, take a long time, are not uniform, and are not guaranteed to be disease-free. Therefore, to meet national banana demand, technology can be utilized to propagate bananas through tissue culture. This study aims to determine the growth response of barangan banana planlets in vitro with the addition of BAP and IBA. The study was carried out at the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University Surakarta from April-December 2024. The method used was a factorial Completely Randomized Design (CRD) consisting of two treatment factors and three replications. The first factor was BAP (0; 1; 2; and 3 ppm) and the second factor was IBA (0; 0.5; 1; and 1.5 ppm). The observation variables included time of shoot emergence, time of root emergence, time of leaf emergence, number of shoots, number of roots, number of leaves, shoot height, and root length. Data analysis was conducted using analisis of varians at a significance level of 95%, if significant continued with Duncan’s Multiple Range Test at a significance level of 95%. The results showed that the combination of BAP and IBA did not affect the number of leaves in Barangan banana plantlets. The combination of BAP 0 ppm and IBA 1.5 ppm produced the best number and length of roots.
