PERTUMBUHAN DAN KANDUNGAN AJMALISIN PADA KULTUR KALUS DAUN TAPAK DARA (Catharanthus roseus (L.) G. Don.) DENGAN PEMBERIAN KINETIN DAN CEKAMAN ASAM

Oleh :
Wiwin Andrias - M0404016 -

ABSTRACT The aim of this research is to study the effect of variation of kinetic and acid stress on growth and ajmalicine content on leaf callus culture Catharanthus roseus (L.) G. Don. The research methods used in this research is factorial experiment 4x4. The first factor is HCl treatment at 4 level of acidity, they are pH 2,9; 3,9; 4,9; and 5,9 as a control. The second factor is variation of kinetic consentration each 0 mg/L as a control; 50 mg/L; 100 mg/L; and 150 mg/L. Each treatment (pH and kinetic combinations) consist of 3 levels. The collected data consist of qualitative data (callus morphology) presented descriptively and quantitative data (fresh weight callus, growht rapid callus, and dry weight callus). The ajmalicine compound tested qualitatively with thin layer chromatography (TLC), then followed the quantitatively test with thin layer chromatography-scanner. Analysis the quantitative data with Analysis of Varian (Anava) then Tamhane research level 5%. The result of research showed that variation of kinetic consentration influence fresh weight callus and growth rapid callus, but didn’t influence dry weight callus. Variation of pH didn’t influence fresh weight callus, growht rapid callus, and dry weight callus. The highest ajmalicine content in callus culture Catharanthus roseus (L.) G. Don., didn’t syncronize with the highest variation of pH consentration and kinetic treatment. The highest ajmalicine content product were in the Murashige Skoog (MS) medium with influence pH 5,9 and kinetic consentration 100 mg/L. Key Word: Growth, Catharanthus roseus, ajmalicine, kinetic, acid stress