UJI TOKSISITAS HASIL FRAKSINASI DAUN RUMPUT MUTIARA (Hedyotis corymbosa (L.) Lamk.) TERHADAP Artemia salina Leach DAN PROFIL KANDUNGAN KIMIA FRAKSI TERAKTIF

Oleh :
Tri Murdiyono - M0404062 -

ABSTRACT Cancer is still become one of the most daunted disease by society at present. But until now it still haven’t been found the way to overcome the disease by satisfied. The other way selected by some patient of this disease by exploiting natural product. One of the plant which have potency for the commodity of traditional drug is Rumput Mutiara ( Hedyotis corymbosa ( L.) Lamk.). In Indonesia this plant has not been used optimally yet as one of the healing form, although the plant has many benefit for healing of various disease, including to cure breast cancer ( Hambali, 2005). The use of this traditional drug requires to be proved scientifically. In this research the toxicity test from fractionation result of Rumput Mutiara (Hedyotis corymbosa ( L.) Lamk.) leaves was conducted by using Brine Shrimp Lethality Test ( BST) method to know its potency as drug of anticancer and compound fraction content on the most active profile. Fractionation method that has been used by Vacuum Liquid Chromatography (VLC). The test has been done by including ten larva A. salina Leach into flacon contain test sample. The mortality percentage of A. salina Leach larva was counted 24 hours after giving of test sample rate series then made equation of linear regression to determine values of LC50-24 hours. The result of toxicity test showed that chloroform extract more toxic than methanol extract. The partition result of chloroform extract showed that soluble fraction of ethyl acetate more toxic than insoluble fraction of ethyl acetate. Dissolve fraction of ethyl acetate was used as toxic fraction, then be fractionated. The test result of each fraction by BST showed that fraction VI is the most toxic with values of LC50-24 hours = 281,77 µg / ml. TLC analysis by various specific reagent showed that compound of alkaloid, flavonoid, terpenoid and ursolic acid was found in the fraction VI. Keyword : Hedyotis corymbosa ( L.) Lamk., Artemia salina Leach, VLC, toxicity