AKTIVITAS ANTIBAKTERI EKSTRAK BUAH PARE BELUT (Trichosanthes anguina L.)

Oleh :
A. RIZAL PERMANA - M0304017 -

The research of antibacterial activity of snake gourd (Trichosanthes anguina L.) extract against pathogenic bacteria have been done. The extracts of snake gourd were prepared by maceration using methanol and then continued using organic solvent with increasing polarity; hexane, chloroform, ethyl acetate, and buthanol, respectively. The antibacterial activity of extracts were determined using diffusion method. Compound analysis of active antibacterial extract was conducted by phytochemical screening. The composition of the highest active extract was investigated using Thin Layer Chromatography, then the minimum inhibitory concentration (MIC) and equivalent value were evaluated. The equivalent value was compared to ampisilin. The result of this research showed that methanol, chloroform and ethyl acetate extracts of snake gourd had antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa, but did not inhibit Salmonella typhii, Shigella dysentriae, and Enterobacter aerogenes. Hexane extract gave an inhibition against E. coli and B. subtilis whereas buthanol extract had antibacterial activity against E. coli, B. subtilis and P. aeruginosa. Chloroform extract had the highest antibacterial activity against S. aureus and P. aeruginosa. The phytochemical screenings analysis showed that active extracts contained alkaloids, tannins, phenolics, flavonoids, and terpenoids compounds. TLC of chloroform extract showed the presence of alkaloids, saponins, tannins, phenolics, flavonoids, and terpenoids. MIC of chloroform extract against S. aureus and P. aeruginosa were 0,0025 mg/μL and 0,00125 mg/μL, respectively. Activity of 0,01 mg/μL chloroform extract compared to ampisilin standard was equal to 2,789.10-6 mg/μL and 2,958.10-5 mg/μL ampisilin for S. aureus and P. aeruginosa, respectively.