Perbandingan efek diuresis air kelapa muda (cocos nucifera l.) Dengan hidroklorotiazid pada tikus putih jantan (rattus norvegicus)

Oleh :
Winda Suryani - G0006167 - Fak. Kedokteran

ABSTRAK Metode penelitian: Penelitian ini bersifat eksperimental laboratorik dengan metode Post Test Only Control Group Design, dilakukan di Laboratorium Farmakologi Universitas Setia Budi. Subjek tikus putih jantan (Rattus norvegicus) strain Wistar, umur antara 2-3 bulan, berat badan 150-200 gram sejumlah 30 ekor. Air kelapa muda diperoleh dari pangkalan kelapa daerah Nusukan. Subjek dibagi menjadi 5 kelompok, masing-masing kelompok perlakuan terdiri 6 ekor tikus. Sebelum perlakuan tikus diadaptasikan selama 7 hari kemudian dipuasakan selama 48 jam dengan tetap diberikan air minum. Kelompok I aquadest 1,8 ml/100 g BB, kelompok II dengan HCT 0,8 ml/100 g BB (0,2 mg/ 100 g BB), kelompok III air kelapa muda 0,9 ml/100 g BB, kelompok IV air kelapa muda 1,8 ml/100 g BB, dan kelompok V air kelapa muda 2,7 ml/100 g BB. Penampungan urin dilakukan selama 4 jam dan pengukuran dilakukan setiap 4 jam selama 16 jam setelah perlakuan. Hasil penelitian: Hasil penelitian menunjukkan volume urin kumulatif 16 jam kelompok I 14,65 ml, kelompok II 76,58 ml, kelompok III 29,57 ml, kelompok IV 38,66 ml, dan kelompok V 59,24 ml. Hasil perhitungan statistik uji Anova dengan α = 0,05 terdapat perbedaan signifikan pada jumlah volume urin antara kelompok I, II, III, IV dan V. Hasil tes post hoc antara kelompok I dengan kelompok yang lain menunjukkan nilai α < 0,05. Hasil kelompok II dibandingkan dengan kelompok III, IV, dan V menunjukkan nilai α < 0,05. Simpulan penelitian: Hasil penelitian ini menunjukkan adanya efek diuresis air kelapa muda pada tikus putih jantan. Air kelapa muda pada ketiga dosis pemberian mempunyai efektifitas diuresis yang tidak sebanding dengan HCT. ABSTRACT Methods: The research is a simple laboratory experimental study using post test only control group design done in pharmacology laboratory of Setia Budi University. The research object is a number of 30 male white rats strain Wistar, aged between 2-3 months old, 150-200 gram of weight. Coconut water was obtained from coconut center in Nusukan. They were divided into five treatment groups, each consisting of six rats. Probability sampling with lottery system has used for sampling technique. Before treatment, rats were adapted for 7 days and fasted for 48 hours but still provided with drinking water. During the treatment the rats were not given drinking water. Coconut water were given orally. Group I were given aquadest 1,8 ml/ 100 g rat weight (BB), group II HCT 0,8 ml/ 100 g rat weight (BB) (0,2 mg/100 g rat weight (BB), group III coconut water dosed I at dosed 0,9 ml/100 g rat weight (BB), group IV coconut water dosed II at dosed 1,8 ml/100 g rat weight (BB), and group V coconut water dosed III at dosed 2,7 ml/100 g rat weight (BB). Rats’ urine was collected for 4 hours and measured its volume every 4 hours for 16 hours after treatment. Results: The result of this study shows that cumulative urine volume in 16 hours for group I 14,65 ml, group II 76,58 ml, group III 29,57 ml, group IV 38,66 ml, and group V 59,24 ml. Result of statistical calculation using anova test with α = 0,05 shows there is a significant difference in total urine volume among group I, II, III, IV, and V. The post hoc test result between group I compared with the others groups was < 0,05. The result between group II compared with group III, IV, and V was < 0,05. Conclusion: The result concludes that there is diuretic effect in the coconut water in male white rats. All doses of the coconut water in this study have diuresis effect inequivalent with HCT.