Pengaruh kurkumin terhadap hitung neutrofil darah tepi pada mencip Balb/C model sepsis paparan cecal inoculum

Oleh :
Dimas wiryantomo - G.0006070 - Fak. Kedokteran

ABSTRACT Sepsis can be caused by delayed apoptosis of neutrophil. Curcumin as an antiinflammatory agent plays role to enhance neutrophil apoptosis. This research is aimed to investigate the effect of curcumin on pheripheral blood neutrophil count in sepsis condition. This was a pure experimental research with post test only control group design. We used 27 male Balb/C mice, 15-30 grams weight, and 4-6 weeks old. They were divided into 3 groups, 9 mice on each group. The groups were control, sepsis model, and sepsis model administered with 1.6 mg curcumin orally. The sepsis model was induced by cecal inoculum 4 mg intraperitoneally. Subjects were treated from day 1 to day 7. On day 8, blood samples were collected from the orbital sinus. Blood neutrophil count was determined by computerization. Statistical analysis was performed by One-Way ANOVA followed by Fisher’s Least Significant Difference (LSD) using SPSS for Windows Release 17.0. Differences were considered significant when p<0.05. The data showed that percentage of neutrophil count of control group (20.86±12.63) %, sepsis (19.44±16.24) %, and sepsis+curcumin (23.63±19.94) %. There is no significant difference between groups (p=0.828). From this research we concluded that there is no effect of curcumin on pheripheral blood neutrophil count in Balb/C mice model of sepsis induced by cecal inoculum. Keywords : curcumin, sepsis, neutrophil, cecal inoculum, apoptosis