Pengaruh rangsang elektroakupunktur terhadap memori kerja tikus putih (rattus novergicus) yang dipapar stres kronik

Oleh :
Muhammad Syaifullah - G0006121 - Fak. Kedokteran

Abstrak Background: Chronic stress may cause morphological changes in the hippocampus followed by deficit in hippocampal function including the impairment of working memory. The aim of this study was to investigate the effect of electroacupuncture (EA) on working memory deficits induced by chronic stress in rats (Rattus norvegicus). Methods: The type of this research is experimental with pretest-postest control group design. The subjects of this study were 24 male rats, divided randomly into four groups (each group consisted of six rats), they were control group (C), stress group (T1), EA group (T2), and EA and stress group (T3). Each group was trained in eight radial arm mazes. T1 group was induced by chronic stress procedure with restraint stress for two hours each day for 21 days. T2 group was given 15 minutes daily EA treatment on Baihui (DU 20) and Zusanli (ST 36) using continous wave at 2 Hz for 14 days. T3 group was induced by chronic stress for 21 days and EA treatment was given for 14 days during stress procedure. Before and after being treated, working memory of each group was tested for 12 days.. Working memory was measured with 8-arm radial maze by the total number of entered arm maze and error A type. The data were analyzed statistically by one-way anova followed by post-hoc test. Result: The results of this study showed that after treatment for each group, T1 group suffered deficits of working memory showed by total number of entered arms maze and error A type for T1 group were higher than C group (p<0.05). There were no working memory difference between T2 group and C group (p>0.05). T3 group had better working memory than T1 group showed by lower total number of entered arms maze and error A type (p<0.05). Conclusion: The conclusion of this study is that chronic restraint stress decrease the working memory in rats. EA treatment on Baihui (DU 20) and Zusanli (ST 36) significantly reverse the working memory deficits in rats after chronic stress. EA treatment in unstressed rats may improve the working memory, but statistically unsignificant