Cytotoxicity Of Combination Extract Pandanus conoideus Lam. Var. Yellow and Red Fruit On mcf-7 Cell

Oleh :
Phyllis Sigrid Pinas - S900809014 - Sekolah Pascasarjana

Many researchers have explored that natural substances have important compound of cancer drug. Variety of red fruit (Pandanus conoideus Lam.) has been extensively used to treat HIV/AIDS, diabetic mellitus, uric acid, osteoporosis and cancer. The sparse evidence leads to an uncertainty whether combination of yellow and red fruit extracts has positive or negative effect. The objectives of this research were to find out the cytotoxic activity of combination of Pandanus conoideus’s yellow and red fruit extract on MCF-7 cell line and the effect of this same extract on MCF-7 breast cancer cell line. Method of Freshney was used in growing MCF-7 cell line. Cytotoxicity test was done to describe the presence of toxic effect on the living cell. Data was analyzed, according to Doyle and Griffith, by calculating the determination of cell death. The data obtained was analyzed using ANOVA followed by DMRT. The calculation of amount of living cell was done with the usage of Doubling time test. Cytotoxicity test results demonstrated that the most active part with a dose of ½ LC BM dan 1LC BK were able to kill 59,9 % MCF-7 breast cancer cells, or showed the highest cytotoxic activity against MCF-7 cells. Concentration with a dose of 0.156: 0.625 μl / ml (BM: BK) proved most effective in inhibiting proliferation, with a longer cell doubling time of 9.135 times (from 11.44 hours to 104.51 hours). Keywords: MCF-7, Pandanus conoideus Lam. var yellow and red fruit extract, cytotoxicity.