Pengaruh diet umbi singkong (Manihot esculenta Crantz) terhadap struktur histologis eksokrin pankreas tikus putih (Rattus Norvegicus)

Oleh :
Adriani Netiasa Suary - G0006034 - Fak. Kedokteran

Tujuan Penelitian: Tujuan penelitian ini adalah untuk mengetahui apakah pemberian diet umbi singkong (Manihot esculenta Crantz) dapat berpengaruh terhadap struktur histologis kelenjar eksokrin pankreas tikus putih (Rattus norvegicus). Metode Penelitian: Penelitian ini bersifat eksperimental laboratorik dengan metode Post Test Only Control Group Design. Penelitian dilakukan di Laboratorium Histologi, Universitas Sebelas Maret, Surakarta. Subjek penelitian adalah 27 ekor tikus putih jantan (Rattus norvegicus) strain Wistar, berumur 12 minggu, berat 150-200 gram, dibagi dalam 3 kelompok, masing-masing 9 ekor. K sebagai kelompok kontrol dengan diet pakan standar ad libitum. P1 diberi diet umbi singkong 100% ad libitum. P2 diberi diet campuran pakan standar dan singkong dengan perbandingan 1:1 ad libitum. Penelitian dilakukan selama 6 minggu. Data yang berupa rerata diameter asinus eksokrin pankreas dianalisis dengan uji statistik One-Way Anova dan dilanjutkan dengan LSD Post Hoc Test, sedangkan data yang berupa peningkatan fibrosis dianalisis menggunakan menggunakan uji statistik Kruskal Wallis dan bila menunjukkan hasil yang signifikan dilanjutkan dengan uji statistik Mann Whitney menggunakan program SPSS for Windows Release 17. Perbedaan dikatakan signifikan bila p < 0,05. Hasil Penelitian: Penelitian ini menunjukkan rerata diameter asinus eksokrin pankreas pada kelompok K yaitu (18,2625 μm), kelompok P1 yaitu (10,7531 μm) kelompok P2 yaitu (19,5507 μm). Terdapat perbedaan yang bermakna antara kelompok K dengan P1 (p=0,000), K dengan P2 (p=0,036), P1 dengan P2 (p=0,000). Pada peningkatan fibrosis didapatkan perbedaan bermakna antara kelompok K dengan P1 (p=0,001) dan P1 dengan P2 (p=0,002) sedangkan pada K dengan P2 tidak didapatkan perbedaan bermakna (p=0,609). Simpulan Penelitian: Pemberian diet singkong dapat mempengaruhi struktur histologis pankreas tikus putih yang ditandai dengan adanya perbedaan rata-rata diameter asinus eksokrin pankreas dan peningkatan fibrosis untuk masing-masing kelompok dibandingkan dengan kelompok kontrol Objective: This reasearch aimed to discover whether or not there was an effect by Cassava (Manihot esculenta) on the histological structure of white rat’s (Rattus norvegicus) pancreas Methods: This research is laboratoric experimental with Post Test Only Control Group Design. It was conducted in the Histology Laboratory of Sebelas Maret University, Surakarta. The subjects of this research were 27 Wistar strain male white rats (Rattus norvegicus) aged twelve months and weiged between 150-200 grams. They were divided into three groups, each of which consisted of nine white rats. K was a control group which given standart diet. P1 was given 100% cassava ad libitum. P2 was given mixed standart diet and choped cassava with a comparison 1:1 ad libitum. This research was done on 6 weeks. For the diameter of acinus exocrine pancreas, data was analysed with One-Way Anova test and was continued with LSD Post Hoc Test and for the increasing of fibrotic tissue, data was analysed with Kruskal Wallis test and continued with Mann Whitney test using SPSS for Windows Release 17 program. The difference were stated significant if the p < 0,05. Results: This research showed the means of diameters of asinus pankreas exocrine. For control group (K) is 18,2625 μm, P1 is 10,7531 μm and P2 is 19,5507 μm. There were significant difference between K group and P1 group (p=0,000), K group and P2 group (p=0,036), P1 group and P2 group (p=0,000). For the increasing of fibrosis, there were significant difference between K group and P1 group (p=0,001), P1 group and P2 group (p=0,002) but there were not significant difference between K group and P2 group (p=0,609). Conclussion: Provision of cassava diet can affect the histological structure of the exocrine pancreas of white rats marked by the diferrences in average diameter of the acini and increasing of fibrotic tissue for each group compared with control group.