Septian Adi Permana, S501102060. 2014. The Differences In The Effect Of Giving Diltiazem Compared To Control In Neutrophille Infiltration Count On Incised Wound Of Wistar Mouse. 1st advisor : Prof Harsono Salimo, Sp. A (K). 2nd advisor : Purwoko, dr., Sp. An, KAKV, KAO. Anesthesiology dan Intensive Therapy Medical Faculty, Post-Graduate Programe, Study Programe Magister of Family Medicine, Sebelas Maret University Surakarta. Background : Postsurgical chronic pain is the consquence of inflammation. Inflammatory processes after surgical incision are maintained through the release of inflammatory mediators by resident cell, which neutrophil is the first appeared. Diltiazem has properties as anti-inflammatoric agent by preventing neutrophil infiltration, and further preventing release of several mediator, including proinflammatory cytokine by neutrophil. Purpose : To identify effect of diltiazem in reduce inflammatory response after surgical incision, base on differentiation of tissue neutrophil infiltration count in rat with and without oral diltiazem. Methods : This is a laboratory setting experiment with randomized post test only control group method. Thirty rats were randomly devided in to two groups. K1, Control group, fiveteen rats with 1 cm skin incision and placebo. K2, fiveteen rats with 1 cm skin incision and oral ketotifen. After the 1st day, the rats were scrified and the tissue suround the wound were taken for hematoxyllin-eosin stainning. The neutrophil infiltration count were analyzed for histologic scoring. Result : it was demonstrated in this study that the histologic score of tissue neutrophil infiltration count of the diltiazem treated group (K2) was significantly lower than control group (K1) (p=0.000). Conclusion : administered of oral diltiazem show signicant differences which is compared to control group. From this research we can also conclude that administering oral diltiazem effective reduce inflammatory response to rat with surgical incision.
