The dosage of pilocarpine to induce status epilepticus in animal model of epilepsy.

Oleh :
Diah Kurnia Mirawati - 196807072003122001 - Fak. Kedokteran

Purpose :
Mechanism of epileptogenicity is remained not well understood until now, although epilepsy has been recognized for several centuries. Research in human being have several limitations, especially in ethics. Therefore animal model was widely used for epilepsy research mainly in field of epileptogenicity mechanism. 
 Pilocarpine is one of several agents can induce status epilepticus in rodent. However, we can not find reference of suitable dose for mice in Indonesia. The purpose of this study is to determine the suitable dosage of pilocarpine to induce status epilepticus in mice.
Method :
This was a preliminary study of doctoral research. We used 32 BALB/c mice for animal model. All of them were male, body weight were 25g – 30g, divided into four groups.  A dosage of 200mg/kg pilocarpine was injected intra peritoneal (ip) into mice group one, 210mg/kg was given into group two, 220mg/kg was given into group three, and 250mg/kg was given into group four. Behavioral seizures were recorded by video camera for six weeks. Decapitation was performed to all mice that survive for six weeks. Mouse brain was proceeded for histopathologic examination.
Result :
There was no evidence of status epilepticus in group one. All of mice in group four got status epilepticus, but all of them died. Six mice in group two got status epilepticus, four of them survived until six weeks. Seven mice in group three got status epilepticus, three of them died during the first day after status epilepticus. Using Kruskal-Wallis test analysis, there was significant difference among groups (p<0>Conclusion :
Suitable dosage of pilocarpine to induce status epilepticus in mice is 220mg.
Keyword :
Pilocarpine – animal model – status epilepticus