Agarwood is a plant that has high economic value, many benefits and is in great demand. The population is increasingly worrying because of the large amount of hunting without cultivation. Tissue culture is one of the way in providing agarwood seedling. This study aimed to determine the effect of concentrations of BAP and organic matter on the growth of agarwood plantlets. The design used was a complete randomized block design (CRBD) with two factors, namely the concentration of BAP at 5 levels (0; 1; 1.5; 2; and 2.5 ppm) and organic matter at 3 levels (without organic matter, tomato extract and bean sprout extract) to obtain 15 treatments with 3 replications. Parameters observed were the time of emergence of buds, number of buds, buds height, and number of leaves. The data were analyzed using analysis of variance (ANOVA) with a level of 5%. If there is a significant effect (sig value > 0.05), then a DMRT (Duncan’s Multiple Range Test) follow-up test was carried out at the 5% level. The results showed that the addition of 1 ppm BAP + tomato extract and 1 ppm BAP + mung bean sprout extract affected the average number of agarwood plantlet leaves. The giving of 2 ppm of BAP was able to increase the emergence time and number of agarwood plantlet buds. The addition of tomato extract and mung bean sprout extract was able to affect the number of leaves and buds height of agarwood plantlets.