Shallots (Allium ascalonicum L.) are a highly demanded commodity in Indonesia. As demand increases, production must also be enhanced to meet this need. One of the superior local varieties of shallots is the Bauji variety from Nganjuk, which has medium-sized bulbs, indicating that its production potential can still be improved. Efforts to increase the production of Bauji shallots can be made through genetic improvement by artificial mutation using the chemical mutagen colchicine. This study aims to examine the performance of the M1V1 generation Bauji shallot variety treated with colchicine and to identify treatments that cause mutations in the Bauji shallot variety. The research was conducted from September to December 2023 in Greenhouse A, Faculty of Agriculture, Sebelas Maret University. This study used a factorial Completely Randomized Design (CRD), with the first factor being colchicine concentration (K) and the second factor being soaking duration (P). The colchicine concentrations used were 0.005% and 0.01%, with soaking durations of 3 hours and 6 hours. The observational data were analyzed using a 5% T-test. The results showed that certain plants indicated polyploidy. The treatment with colchicine concentration of 0.01% and 3 hours soaking (K2P1) is suspected to be the most effective in causing mutations in the Bauji variety of shallots.