Red betel plant is a plant with high economic value because it’s used by the community as a medicinal plant. Problems that often occur in the cultivation of red betel plant are inappropriate environmental conditions and growing media so that plants are in a stressed condition. This is because inappropriate environmental conditions will affect physiological processes in plants. The improper composition of the growing media will also inhibit root growth it affect the growth and yield of red betel plant. This study aims to obtain the composition of growing media and the concentration of vitamin B1 that increases the growth and yield of red betel plant. The study used a factorial Randomized Completely Block Design with two factors, namely growing media (soil: manure (1:1), soil: manure: husk charcoal (1:1:1), soil: manure: husk charcoal (1:1:2), soil: manure: husk charcoal (2:1:1)) and vitamin B1 concentration (0,2,4 ml/l). Data were analyzed with an ANOVA of 5% level, if it showed significance, it was continued with DMRT and regression test. The results of the study showed that there was an interaction between soil, manure, husk charcoal (1:1:1) and vitamin B1 concentration of 4 ml/l which gave the best results on flavonoid levels which is 1.97%. The composition of soil, manure, and husk charcoal (1:1:2) gave the best results in the variables of plant height (118,97 cm), number of leaves (20,88 sheet), length of internodes (7,51 cm), leaf area (2911,95 cm2), root length (26,63 cm), dry weight of leaves (3,25 gram), dry weight of crops (6,06 gram), fresh weight of leaves (19,11 gram), and fresh weight of crops (38,76 gram). Vitamin B1 concentrations alone didn’t increase the growth and yield of red betel plant.