Centella asiatica DNA Barcoding: Assessing Antibacterial Potential and Environmental Factors
Penulis Utama
:
Lamlom Taie Lamlom Tahawy
NIM / NIP
:
S902208003
×<p><div xss=removed msttexthash="389547509" msthash="374"><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>Centella asiatica</span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>, often known as Gotu Kola, has a rich and extensive history in the field of traditional medicine. Recent experiments have shown that these plants have the potential to be reservoirs of antibacterial compounds, specifically those targeting </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>MRSA</span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>. The antibacterial properties </span><span xss=removed> of </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>C. asiatica </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>from various regions are still not adequately characterized. This thesis examines the genetic diversity, bioactive component profile, and antibacterial activity </span><span xss=removed> of </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>C. asiatica </span><span xss=removed> populations </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>in Tawangmangu, Central Java, Indonesia. The study aims to identify populations that fight MRSA and review the impact of environmental variables on genetic diversity and plant bioactivity. </span><span class="fontstyle3" mstmutation="1" istranslated="1" xss=removed>Methods: </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>DNA barcodes with the rbcL gene were used to study genetic diversity in samples from Kemuning, Sekipan, and Taman Hutan Rakyat. Antimicrobial activity against </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>MRSA </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>was evaluated using</span></div><font mstmutation="1" msttexthash="2109210805" msthash="352"><div xss=removed msttexthash="121201860" msthash="373"><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>disc diffusion. The effects of pH, light intensity, humidity, and temperature on the synthesis of bioactive chemicals were studied. </span><span class="fontstyle3" mstmutation="1" istranslated="1" xss=removed>Results: </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>DNA barcoding revealed significant genetic diversity, with Kemuning showing distinct genetic lineages and People's Forest Park showing divergence. The environment significantly influences the synthesis of saponins and terpenoids, with the People's Forest Park showing the highest amounts. However, no extract from </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>C. asiatica </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed msttexthash="207259" msthash="378">was effective </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>in reducing </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>MRSA </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>at the amounts studied. </span><span class="fontstyle3" mstmutation="1" istranslated="1" xss=removed msttexthash="648271" msthash="381">Antimicrobial potency test: </span><span xss=removed msttexthash="13486564" msthash="380">Lack of antimicrobial agent amounts, inadequate extraction methods, orgenetic variations affecting the synthesis of these compounds may explain the lack </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>of antibacterial effectiveness against </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>MRSA</span><span class="fontstyle2" mstmutation="1" istranslated="1" msttexthash="4438889" msthash="382" xss=removed>, despite the presence of saponins and terpenoids. The study of medicinal plants such as </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>C. asiatica </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed msttexthash="233441" msthash="384">requires a deep </span><span xss=removed msttexthash="4318821" msthash="383">understanding of genetic diversity and environmental conditions. The next research </span><span xss=removed msttexthash="3608163" msthash="385">goal should be to improve extraction methods, learn more about the genetics of </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>the synthesis of bioactive components, and discover the environmental factors that help </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed msttexthash="10881" msthash="387">C. </span><span class="fontstyle0" mstmutation="1" istranslated="1" xss=removed>asiatica </span><span class="fontstyle2" mstmutation="1" istranslated="1" xss=removed>make more antibacterial substances</span></div></font></p>
×
Penulis Utama
:
Lamlom Taie Lamlom Tahawy
Penulis Tambahan
:
1. Dr. Ari Susilowati, M.Si 2. Prof. Drs. Sutarno, M.Sc., Ph.D 3.
NIM / NIP
:
S902208003
Tahun
:
2024
Judul
:
Centella asiatica DNA Barcoding: Assessing Antibacterial Potential and Environmental Factors
Edisi
:
Imprint
:
Surakarta (Kota) - Fak. MIPA - 2024
Program Studi
:
S-2 Biosains
Kolasi
:
Sumber
:
Kata Kunci
:
C. asiatica , antibacterial , MRSA , barcoding DNA
