Dioscorea alata has a low crossing success rate and is also faced with erratic climate problems, making it difficult for farmers to cultivate this plant. One alternative that can be done to propagate D. alata species is by tissue culture. This study aims to obtain the best type and concentration of cytokinin to induce shoots of D. alata explants. The research was conducted at the In Vitro Laboratory, Departement of Agriculture, Universiti Putra Malaysia, from December 2023 - February 2024. The medium used in this study was Murashige and Skoog with NAA as growth regulator combined with various types of cytokinins, namely BAP, kinetin, and 2iP. The method used was a Completely Randomized Design (CRD) non factorial with 10 treatment combinations and four replications (Without Plant Growth Regulator (PGR), NAA 3 μM.L-1 + BAP 10 μM.L-1, NAA 3 μM.L-1 + Kinetin 10 μM.L-1, NAA 3 μM.L-1 + 2iP 10 μM.L-1, NAA 3 μM.L-1 + BAP 15 μM.L-1, NAA 3 μM.L-1 + Kinetin 15 μM.L-1, NAA 3 μM.L-1 + 2iP 15 μM.L-1, NAA 3 μM.L-1 + BAP 20 μM.L-1, NAA 3 μM.L-1 + Kinetin 20 μM.L-1, NAA 3 μM.L-1 + 2iP 20 μM.L-1). The variables observed in this study were plant height, number of shoots, shoot length, number of nodes, and number of leaves. Data analysis used analysis of variance (ANOVA) with 5% error rate then continued with Duncan’s Multiple Range Test (DMRT) with 5% error rate. The results showed that application of various types of cytokinins had an effect on the growth of D. alata explants. The application of BAP, kinetin, and 2iP has a significant effect on plant height, number of shoots, shoot length, number of nodes, and number of leaves of D. alata explants. The application of NAA 3 μM.L-1 + 2iP 20 μM.L-1 to the media provides the best growth with significant results than other cytokinins.