PERTUMBUHAN TUNAS PURWACENG (Pimpinella alpina K. D. S.) PADA PERLAKUAN PRAKONDISI DENGAN VARIASI KONSENTRASI THIDIAZURON
Penulis Utama
:
Beny Nur Fitriani
NIM / NIP
:
M0403013
×ABSTRACT
Purwaceng (Pimpinella alpina K. D. S.) is one of endangered species which is
potential to be developed as aphrodisiac source. To support conservation and cultivation
of purwaceng, it is needed to prepare the plant material. Tissue culture technique is one
elternative methode for both cultivation and regeneration. Constraint in micropropagation
of purwaceng are the limited explant source, high level of senescence and low level of
shoot multiplication
The aims of this research were to increase shoot growth by in vitro
preconditioning on Driver and Kuniyuki Walnut (DKW) medium with various
consentration of Thidiazuron (TDZ) (0 mg/l; 0.5 mg/l; 1 mg/l; 1.5 mg/l; 2 mg/l). The
cultures were incubated in preconditioning medium for 2 weeks, then they were
subcultured on DKW basal medium for 8 weeks. Wet and dry weight, lenght and also
visual performance of shoot were observed.
During incubation in preconditioning medium, there was no senescence on shoot.
Observation after 10 weeks incubation, showed that the treatment has no significant
effect on shoot growth, but succes to delayed senescence. Wet weight, dry weight and
length of shoot at preconditioning medium were generally lower than control.
Precondition with TDZ at consentration 0 mg/l to 1.5 mg/l increased biomass
production of purwaceng culture wich indicated by increasing wet and dry weight, and
decreasing at consentration 2 mg/l of TDZ. The length of shoot increased at consentration
0 mg/l to 1 mg/l, but it decreased at consentration 1.5 mg/l and increased again at
consentration 2 mg/l of TDZ. The highest wet and dry weight was showed by treatmen at
1.5 mg/l TDZ, but lowest on lenght of shoot.
Keywords: Pimpinella alpina K. D. S., in vitro, preconditioning, thidiazuron, shoot
growth.
×
Penulis Utama
:
Beny Nur Fitriani
Penulis Tambahan
:
-
NIM / NIP
:
M0403013
Tahun
:
2008
Judul
:
PERTUMBUHAN TUNAS PURWACENG (Pimpinella alpina K. D. S.) PADA PERLAKUAN PRAKONDISI DENGAN VARIASI KONSENTRASI THIDIAZURON
