TRANSFORMASI GEN XILOGLUKANASE PADA BEBERAPA JENIS EKSPLAN SENGON (Paraserianthes falcataria L. Nielsen) MELALUI Agrobacterium tumefaciens
Penulis Utama
:
Tri Warseno
NIM / NIP
:
M0403010
×ABSTRACT
Sengon (Paraserianthes falcataria L. Nielsen) is one of forestry crop
which have been developed and recommended for Industrial Timber Estate
(HTI). The economic value of this plant such as pulp, raw material of paper
industry (pulp and paper), construction, plywood, and also for energy was
widely known. To increase the wood quality of sengon in industry especially
for the pulp, sengon plantation in which consist higher cellulose were
required. Genetic engineering with the DNA technology for the crop
improvement in which the times taken where much shorten compare to
conventional one. Overexpression of xyloglucanase in poplar (Populus alba)
showed the growth enhancement and improved the cellulose depotition at
secondary xylem so wood quality yielded good progressively. The aim of this
research was to know the result of xyloglucanase gene transformation via
Agrobacterium tumefaciens vector of various explants type of sengon. Callus, cotyledons and cotyledonary nodes were taken away from
sengon’s seed which germinated on hormon-free MS medium during 10 days
used for transformation. A. tumefaciens carrying recombinant plasmid
pAaXEG 300 bearing xyloglucanase and NPT II gene encoding kanamycin
resistance were used for transformation process. For cocultivation A.
tumefaciens with Optical Density (OD600) = 0,4; 0,6 and 0 (as control) value
was used. For callus induction 1mg TDZ and 0,25 mg/IAA were added to MS
medium respectively. For the selection purpose 300 mg/l kanamycin and 280
mg/l carbenisillin were added to MS medium. The putative transgenic
explants were tested on a gene integration test by PCR methode and the results
was checked by gel electroforesis. Putative transgenic explants then moved to
the somatic embryo induction medium to induce somatic embryo forming.
The results showed that the OD600 0,4 value showed much better than
OD600= 0,6 for the all of explants used, accordingly callus yielded highest
transformation efficiency value ( 40 %). PCR products examined, resulting
showed that six of the nine samples tested showed positif, indicating that
xyloglucanase gene has successfully integrated on the plant tissue.
Key words: xyloglucanase, Paraserianthes falcataria, explant types,
transformation
×
Penulis Utama
:
Tri Warseno
Penulis Tambahan
:
-
NIM / NIP
:
M0403010
Tahun
:
2008
Judul
:
TRANSFORMASI GEN XILOGLUKANASE PADA BEBERAPA JENIS EKSPLAN SENGON (Paraserianthes falcataria L. Nielsen) MELALUI Agrobacterium tumefaciens
Edisi
:
Imprint
:
SURAKARTA - FMIPA - 2008
Program Studi
:
S-1 Biologi
Kolasi
:
Sumber
:
Kata Kunci
:
Jenis Dokumen
:
Skripsi
ISSN
:
ISBN
:
Link DOI / Jurnal
:
-
Status
:
Public
Pembimbing
:
1. Prof. Drs. Suranto,M.Sc., Ph.D. 2. Dra. N. Sri Hartati, M.Si.
Penguji
:
Catatan Umum
:
Fakultas
:
×
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:
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