PENGARUH KOMBINASI BAP (Benzil Amino Purine) DAN NAA (Naphtalene Acetic Acid) TERHADAP PERTUMBUHAN TUNAS ZODIA (Euodia suaveolens Scheff.) SECARA IN VITRO
Penulis Utama
:
Eni Sugiyanti
NIM / NIP
:
M0403026
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Using plant growth regulators (PGRs) in the appropriate concentrations
and combination can increase the growth of shoot. The study has aims to evaluate
the effect of combinant concentration of plant growth regulator of BAP and NAA,
and determine the optimal concentration of BAP and NAA to support the growth
and development shoots of zodia (Euodia suaveolens Scheff.) by in vitro.
The experiment was arranged in Completed Randomized Design with six
treatment that are combination: 0 mg/l BAP + 0 mg/l NAA (control), 1 mg/l BAP
+ 1 mg/l NAA, 2 mg/l BAP + 1 mg/l NAA, 3 mg/l BAP + 1 mg/l NAA, 4 mg/l
BAP + 1 mg/l NAA, 5 mg/l BAP + 1 mg/l NAA each treatment with four
replicates. The explans is axilar shoot’s from plants in the age of 3th
month. The
cultures were then incubated at 25-280
C under a 12-hour photoperiod with
provided by cool white fluorescent tubes (TL 10 W) for 5 weeks. Parameter that is
observed is a morphology of callus, time of appearing shoot, number of shoot,
height of shoot, and morphology of shoot.
The result of the study proves that the explants cultured in an standar MS
media supplemented with combination treatment affected in vitro growth or
regeneration of Zodia shoots. Shoots successfully grew and developed. The Callus
was formed on the of all treatment except on control. At the second week 2 mg/l
BAP and 1 mg/l NAA showed the most bud (1,75). The highest bud number was
5,75 pieces with a combination of BAP 3 mg/l and NAA 1 mg/l, while the highest
bud height (9,8 mm) was obtained on control.
Key words: in vitro culture, Euodia suaveolens Scheff., shoot’s growth, BAP,
NAA.
×
Penulis Utama
:
Eni Sugiyanti
Penulis Tambahan
:
-
NIM / NIP
:
M0403026
Tahun
:
2008
Judul
:
PENGARUH KOMBINASI BAP (Benzil Amino Purine) DAN NAA (Naphtalene Acetic Acid) TERHADAP PERTUMBUHAN TUNAS ZODIA (Euodia suaveolens Scheff.) SECARA IN VITRO
Edisi
:
Imprint
:
SURAKARTA - FMIPA - 2008
Program Studi
:
S-1 Biologi
Kolasi
:
Sumber
:
Kata Kunci
:
Jenis Dokumen
:
Skripsi
ISSN
:
ISBN
:
Link DOI / Jurnal
:
-
Status
:
Public
Pembimbing
:
1. Solichatun, M.Si. 2. Widya Mudyantini, M.Si.
Penguji
:
Catatan Umum
:
Fakultas
:
Fak. MIPA
×
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:
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