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<p class="MsoNormal" xss=removed><span lang="EN-US" xss=removed>Helmi Susanti Simamora. S532208020. 2024 <b>Pengaruh Ekstrak Kelakai </b></span><b><span lang="ZH-CN" xss=removed>(<i>Stenochlaena</i></span></b><b><i><span lang="EN-US" xss=removed><span xss=removed> </span></span></i></b><b><i><span lang="ZH-CN" xss=removed><span xss=removed> </span>Palustris</span></i></b><b><i><span lang="ZH-CN" xss=removed> </span></i></b><b><i><span lang="ZH-CN" xss=removed>(Burm.) Bedd</span></i></b><b><span lang="ZH-CN" xss=removed>) </span></b><b><span lang="EN-US" xss=removed>Terhadap Kadar Glukosa Darah, ROS, </span></b><span lang="ZH-CN"><a href="https://www.nafiriz.com/p/skripsi-ub.html?cx=partner-pub-3f2f393f313395f72&cof=FORID:10&ie=ISO-8859-1&q=Pengaruh Terapi Ekstrak Blueberry (Vaccinium corymbosum) Terhadap Ekspresi TNF-α Ginjal dan Profil Protein Hati pada Tikus (Rattus norvegicus) Glomerulonefritis Hasil Induksi Streptokinase"><b><span lang="EN-US" xss=removed>SOD</span></b></a></span><b><span lang="EN-US" xss=removed> dan Kortisol </span></b><b><span lang="EN-US" xss=removed>Pada </span></b><b><span lang="ZH-CN" xss=removed>Tikus</span></b><b><span lang="EN-US" xss=removed> Jantan </span></b><b><span lang="ZH-CN" xss=removed>Model Diabetes
Melitus Tipe </span></b><b><span lang="EN-US" xss=removed>2. </span></b><span lang="EN-US" xss=removed>Tesis. Pembimbing I: </span><span lang="ZH-CN" xss=removed>Prof. Dr. Adi
Prayitno, drg., M.</span><span lang="ZH-CN" xss=removed> </span><span lang="ZH-CN" xss=removed>Kes</span><span lang="EN-US" xss=removed>., </span><span lang="EN-US" xss=removed>Sp.PMM(K).</span><span lang="EN-US" xss=removed> Pembimbing II: </span><span lang="ZH-CN" xss=removed>Dr. Muthmainah,
dr. M.</span><span lang="ZH-CN" xss=removed> </span><span lang="ZH-CN" xss=removed>Kes</span><span lang="EN-US" xss=removed>. Program Studi Ilmu Gizi, Sekolah PascaSarjana Universitas Sebelas
Maret. </span></p>
<p class="MsoNormal" xss=removed><span lang="EN-US" xss=removed> </span></p>
<h1><a name="_Toc183500621"></a><a name="_Toc185181099"><span xss=removed><b><span lang="ZH-CN" xss=removed>ABSTRAK</span></b></span></a><span xss=removed></span><span xss=removed></span><b><span lang="ZH-CN" xss=removed></span></b></h1>
<p class="MsoNormal" xss=removed><b><span lang="ZH-CN" xss=removed>Latar Belakang Masalah</span></b><span lang="ZH-CN" xss=removed>:</span><span lang="EN-US" xss=removed>Diabetes melitus setiap tahunnya meningkatkan,<span xss=removed> </span>hiperglikemi dapat menyebabkan terjadinya stress
oksidatif sehingga terjadinya peningkatan ROS, penurunan SOD<i> dan </i>peningkatan
kadar kortisol. Senyawa aktif yang ada pada kelakai </span><span lang="ZH-CN" xss=removed>seperti flavonoid,
alkaloid, fenol yang bermanfaat bagi </span><span lang="EN-US" xss=removed>k</span><span lang="ZH-CN" xss=removed>esehatan</span><span lang="EN-US" xss=removed>. </span><b><span lang="ZH-CN" xss=removed>Tujuan </span></b><span lang="EN-US" xss=removed>membuktikan pengaruh ekstrak batang dan daun kelakai terhadap kadar glukosa
darah sewaktu (GDS), R<i>eactive Oxygen Species<span xss=removed>
</span></i>(ROS), <i>Superoxide Dismutase </i>(SOD) dan kortisol pada tikus
jantan model diabetes melitus tipe 2. </span><b><span lang="ZH-CN" xss=removed>Metode</span></b><span lang="ZH-CN" xss=removed>: </span><span lang="EN-US" xss=removed>Penelitian ini adalah </span><span lang="ZH-CN" xss=removed>eksperimen
laboratori</span><span lang="EN-US" xss=removed>k dengan </span><i><span lang="ZH-CN" xss=removed>pre</span></i><span lang="ZH-CN" xss=removed> and <i>post</i>test
<i>kontrol group design</i></span><span lang="EN-US" xss=removed>. B</span><span lang="EN-US" xss=removed>erat badan 220 – 280 gr, jumlah sampel tikus
jantanWistar 25 ekor yang dibagi menjadi 5 kelompok perlakuan: KN (kelompok
normal), K- (tikus diabetes), K+ (tikus DM+metformin 45mg/kgBB), P1 dan P2
tikus DM masing-masing kelompok diberi ekstrak kelakai dengan dosis 400 dan 800
mg/kgBB selama 21 hari, diberikan melalui sonde setiap jam 08.00-09.00.</span><span lang="EN-US" xss=removed> </span><span lang="EN-US" xss=removed>Pembuatan model DM dengan menggunakan </span><em><span lang="ZH-CN" xss=removed>streptozotosin</span></em><span lang="EN-US" xss=removed> (STZ) dan </span><em><span lang="ZH-CN" xss=removed>nikotinamid</span></em><span lang="EN-US" xss=removed> (NA) melalui </span><em><span lang="ZH-CN" xss=removed>intraperitoneal</span></em><span lang="ZH-CN" xss=removed> </span><span lang="EN-US" xss=removed>(IP) selama 3 hari.</span><span lang="EN-US" xss=removed> </span><span lang="EN-US" xss=removed>Pembuatan
ekstrak batang dan daun kelakai menggunakan metode maserasi, dengan pelarut
etanol 70%. Pemeriksaan GDS dengan menggunakan <i>glucometer </i>(Autocheck).
Pemeriksaan MDA, SOD dan Kortisol menggunakan ELISA. Data dianalisis dengan uji
<i>One Way Anova</i> untuk pemeriksaan GDS dengan nilai <i>signifikan</i> <i>p</i>=
0,03, kadar ROS <i>p</i>= 0,022. Kadar SOD <i>p</i>= 0,042 dan kadar kortisol <i>p</i>=0,035.
<b>Hasil</b>: Ekstrak batang dan daun kelakai mengandung fitokimia, flavonoid
76,727 ppm, alkaloid = 86,524 ppm, fenol = 56,180 mg GAE, aktifitas antioksidan
= 78,787 ppm. Kadar GDS pada kelompok P1 lebih rendah secara signifikan
dibandingkan K- (p=0,047), kadar MDA pada kelompok P1 (0,010) dan P2 (0,006)
lebih rendah secara signifikan, kadar SOD pada kelompok P1 lebih tinggi secara
signifikan dibandingkan K- (p=0,020), kadar Kortisol pada kelompok P2 lebih rendah
secara signifikan dibandingkan K- (p=0,046).</span><span lang="EN-US" xss=removed> </span><b><span lang="EN-US" xss=removed>Kesimpulan</span></b><span lang="EN-US" xss=removed>: Pemberian ekstrak kelakai dengan dosis 400mg/kg
dapat menurunkan kadar GDS, menurunkan kadar MDA dengan dosis 400mg/kgBB dan
800mg/kgBB, meningkatkan kadar SOD dengan dosis 400mg/kgBB dan menurunkan kadar
kortisol pada dosis 800 mg/kgBB.</span></p>
<p class="MsoNormal" xss=removed><span lang="EN-US" xss=removed> </span></p>
<p class="MsoNormal" xss=removed><span lang="EN-US" xss=removed>Kata Kunci: Ekstrak batang dan daun kelakai; diabetes
melitus; Kortisol; MDA; SOD</span></p>
<p class="MsoCommentSubject" xss=removed><span lang="EN-US" xss=removed> </span></p>
<h1><a name="_Toc183500621"></a></h1><br>
×
Penulis Utama :
Helmi Susanti Simamora
Penulis Tambahan :
-
NIM / NIP :
S532208020
Tahun :
2025
Judul :
Pengaruh Ekstrak Kelakai (Stenochlaena Palustris (Burm.) Bedd) Terhadap Kadar Glukosa Darah, Ros, Sod Dan Kortisol Pada Tikus Jantan Model Diabetes Melitus Tipe 2
Edisi :
Imprint :
Surakarta - Sekolah Pascasarjana - 2025
Program Studi :
S-2 Ilmu Gizi
Kolasi :
Sumber :
Kata Kunci :
Ekstrak batang dan daun kelakai; diabetes melitus; Kortisol; MDA; SOD
Jenis Dokumen :
Tesis
ISSN :
ISBN :
Link DOI / Jurnal :
-
Link DOI :
-
Status :
Public
Pembimbing :
1. Prof. Dr. Adi Prayitno, drg., M. Kes
Penguji :
1. Dono Indarto, dr., M.Biotech.St, Ph.D., AIFM
Catatan Umum :
Fakultas :
Sekolah Pascasarjana
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