The decline in paddy production occurs due to changes in environmental conditions due to excessive use of chemicals, one of which is the use of chemical fertilizers. Efforts that can be made are the use of organic materials to improve environmental conditions and reduce the use of chemical fertilizers. This research aims to determine the effect of chemical fertilizer substitution with the provision of biological fertilizers Bacillus, Nitrobacter, Liquid Organic Fertilizer (LOF) Amino Acids and Silica on the growth and yield of paddy. The research was conducted in the paddy fields of Segawe Hamlet, Purwosari Village, Wonogiri District, Wonogiri Regency, Central Java from April-August 2024. The method used was a field experiment using a single non-factorial plot of six treatment plots without replication in the form of control treatment, biological fertilizers (Bacillus and Nitrobacter) + LOF amino acids + silica; added 25; 50; 75; and 100% chemical fertilizer. The observed variables were plant height, number of tillers, number of panicles, number of grains, fresh and dry grain weight, percentage of full grains, number of 1000 grains, and dry grain yield (GKP). The research data were analyzed using a t-test at a 95% significance level. If the results are significant, they are followed up with a Pearson correlation test at a 95% significance level. The results showed that the combination fertilization treatment of biological fertilizers (bacillus, nitrobacter) + LOF amino acids + silica fertilizer with 100% chemical fertilizer gave the highest results in plant height, leaf greenness level and number of panicles. combination fertilization treatment of biological fertilizer with 100% chemical fertilizer gave the highest results in tillers per hill, percentage of full grain, weight of 1000 grains and weight of dry harvested grain.