Sambiloto was a medicinal plant, had seen increased market demand but not production, posing a threat to its wild germplasm. To increase sambiloto production and maintain its germplasm, cultivation efforts were needed such as sunlight intensity and water intensity. This study aimed to determine the optimal combination of sunlight intensity, watering time interval, and the level of sunlight intensity for sambiloto growth. The research was conducted in Tempel Village, Bendosari Sub-district, Sawit District, Boyolali Regency, and continued at the Faculty of Agriculture Sebelas Maret University Laboratory from July-November 2023. The method used was a Completely Randomized Design (CRD) with Split Plot Design. The first factor was light intensity as main treatment (100; 50; and 25%) and the second factor was watering time interval (2, 3, and 4 days). Variables observed were plant height, number of leaves, number of branches, leaf area, fresh weight of stash (roots, stems and leaves), dry weight of stash (roots, stems and leaves), stem length, root length and chlorophyll content. Data were analyzed using ANOVA (Analysis of Variance) at a 5% error level, and if significant differences were found, the analysis was continued with DMRT (Duncan’s Multiple Range Test) at the 5% error level, along with correlation analysis. The result showed that watering time interval every 2 days had a significant effect on all growth variables and plant yield variables. There was an interaction between the treatment of sunlight intensity and watering time intervals on the number of sambiloto branches. However, further research needed to determine the treatment given to the content of secondary metabolite content of sambiloto.