Pengaruh rangsang elektroakupunktur terhadap memori kerja tikus putih (rattus novergicus) yang dipapar stres kronik
Penulis Utama
:
Muhammad Syaifullah
NIM / NIP
:
G0006121
×Abstrak
Background: Chronic stress may cause morphological changes in the
hippocampus followed by deficit in hippocampal function including the
impairment of working memory. The aim of this study was to investigate the
effect of electroacupuncture (EA) on working memory deficits induced by chronic
stress in rats (Rattus norvegicus).
Methods: The type of this research is experimental with pretest-postest control
group design. The subjects of this study were 24 male rats, divided randomly into
four groups (each group consisted of six rats), they were control group (C), stress
group (T1), EA group (T2), and EA and stress group (T3). Each group was trained
in eight radial arm mazes. T1 group was induced by chronic stress procedure with
restraint stress for two hours each day for 21 days. T2 group was given 15 minutes
daily EA treatment on Baihui (DU 20) and Zusanli (ST 36) using continous wave
at 2 Hz for 14 days. T3 group was induced by chronic stress for 21 days and EA
treatment was given for 14 days during stress procedure. Before and after being
treated, working memory of each group was tested for 12 days.. Working memory
was measured with 8-arm radial maze by the total number of entered arm maze
and error A type. The data were analyzed statistically by one-way anova followed
by post-hoc test.
Result: The results of this study showed that after treatment for each group, T1
group suffered deficits of working memory showed by total number of entered
arms maze and error A type for T1 group were higher than C group (p<0.05).
There were no working memory difference between T2 group and C group
(p>0.05). T3 group had better working memory than T1 group showed by lower
total number of entered arms maze and error A type (p<0.05).
Conclusion: The conclusion of this study is that chronic restraint stress decrease
the working memory in rats. EA treatment on Baihui (DU 20) and Zusanli (ST 36)
significantly reverse the working memory deficits in rats after chronic stress. EA
treatment in unstressed rats may improve the working memory, but statistically
unsignificant
