The C-Reactive Protein (CRP) and Matrix Metalloproteinase-1 (MMP-1) Serum level in Preterm Premature Rupture of Membranes

Gustavry, Abraham Andy

× Abraham Andy Gustavry, S 501002027, 2014, The C-Reactive Protein (CRP) and Matrix Metalloproteinase-1 (MMP-1) Serum level in Preterm Premature Rupture of Membranes, Advisor : DR. Sri sulistyowati, dr, SpOG (K), Co-advisor : DR. Supriyadi Hari Respati, dr, SpOG, Thesis, Magister of Family Medical Doctor Program, Post Graduate Program, Sebelas Maret University, Surakarta Preterm Premature rupture of membranes (PPROM) is a major obstetric problem associated with complications in labor that increase maternal and perinatal mortality and morbidity. Elevated C-Reactive Protein (CRP) in maternal circulation is associated with the presence of intra uterine infection. C-Reactive Protein (CRP) is an acute-phase reactant protein synthesized primarily by liver cells in respon to the proinflammatory cytokines. Thus, elevated of CRP will stimulated MMP-1 release. Matrix metalloproteinases (MMPs) are contribute in extracellular matrix (ECM) remodeling/degradation leading to fetal membrane rupture. MMP-1 degrade fibrillar collagen types I, II and III which play crucial role in PPROM Aim: to investigate the levels of hs-CRP and MMP-1 serum in PPROM pregnancy and normal pregnancy. Study Design: a descriptive cross sectional analytic approach with clinical trial levels of serum CRP and MMP-1 with ELISA method. The study was conducted in November - December 2013 in the obstetrics and gynecology department of Moewardi Hospital Surakarta and Prodia Laboratory Jakarta. 40 subjects of a qualified acceptance of the sample (inclusion and exclusion criteria) was examined and divided by 2 groups which are 20 subjects with PPROM and 20 subjects as control. Further, examinations were done to know serum levels of CRP and MMP-1 which was then tested statistically using t- test and regression analysis. Result: 40 subjects consist of 20 subjects with preterm PROM and 20 sbujects normal pregnant as control. Data obtained from these subjects that were the mean serum levels of CRP in PPROM pregnant patients were (13,17 ± 14,69 mg/L) and normal pregnant patients were (4,21 ± 2,82 mg/L) with the results of the t-test p value : 0,00 (p <0.05). It also obtained that the mean serum levels of MMP-1 in PPROM pregnancy patients were (7,68 ± 1,63 ng/ml) and normal pregnant patients were (6,23 ± 0,84 ng/ml) with the results of the t-test p value : 0,00 (p <0.05). Since there are differences in the increase of PPROM, test was followed by multivariate linear regression analysis. The Pearson correlation test resulted an increase in hs-CRP and MMP-1 were obtained p-value: 0,00 (p <0.05) with a correlation coefficient of 0,841 indicates that there was a positive correlations relationship with medium correlation between elevated levels of CRP to the increased levels of MMP-1. Conclusion: levels of serum CRP and MMP-1 in PPROM pregnancy is higher than normal pregnancy. Causative relationship was exist that increased CRP also resulted in increased MMP-1 (84,1%) thus increasing both levels affect the PPROM pregnancy. Keywords : Preterm Premature Rupture of Membranes, hs-CRP, MMP

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Penulis Utama	:	Abraham Andy Gustavry
Penulis Tambahan	:	-
NIM / NIP	:	S50100202
Tahun	:
Judul	:	The C-Reactive Protein (CRP) and Matrix Metalloproteinase-1 (MMP-1) Serum level in Preterm Premature Rupture of Membranes
Edisi	:
Imprint	:	- -
Program Studi	:	S-2 Biomedik
Kolasi	:
Sumber	:
Kata Kunci	:
Jenis Dokumen	:	Tesis
ISSN	:
ISBN	:
Link DOI / Jurnal	:	-
Status	:	Public
Pembimbing	:	1. DR. Sri sulistyowati, dr, SpOG (K) 2. DR. Supriyadi Hari Respati, dr, SpOG
Penguji	:
Catatan Umum	:
Fakultas	:	Sekolah Pascasarjana

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