ABSTRAKLatar Belakang: Kolesterol Low Density Lipoprotein (LDL) berhubungan denganPenyakit Jantung Koroner (PJK) yang merupakan salah satu penyebab kematianutama di dunia. Buncis mengandung flavonoid, phytosterol, saponin, niasin,vitamin C, dan serat yang memiliki efek hipolipidemia. Penelitian ini bertujuanuntuk mengetahui pengaruh ekstrak buncis terhadap kadar kolesterol LDL tikusputih model hiperkolesterolemia.Metode: Penelitian ini bersifat eksperimental laboratorik dengan desain the posttestonly control group design, dilakukan di Laboratorium Histologi FakultasKedokteran Universitas Sebelas Maret. Sampel sebanyak 30 ekor tikus putih(Rattus norvegicus), jantan, galur Wistar. Kelompok Kontrol (KK) diberi pelet danakuades selama 28 hari. Kelompok Perlakuan 1–4 (KP1-4) diberi kuning telur bebek5 gram/200 gram BB tikus putih/hari dan PTU 0,01% selama 28 hari. Hari ke 15 –28, KP2 diberi simvastatin 0,72 mg/200 gram BB tikus putih/hari, KP3 diberiekstrak buncis 100 mg/200 gram BB tikus putih/hari dan KP4 diberi ekstrak buncis150 mg/200 gram BB tikus putih/hari. Data kadar kolesterol LDL dianalisis denganuji One-way ANOVA yang dilanjutkan dengan Post Hoc Test.Hasil: Hasil pengukuran kadar kolesterol LDL didapatkan nilai rerata pada KK =33.83 ± 14.442 mg/dl, KP1 = 54.00 ± 27.943 mg/dl, KP2 = 18.83 ± 7.672 mg/dl,KP3 = 77.33 ± 20.954 mg/dl dan KP4 = 80.17 ± 31.923 mg/dl. Analisis statistikdengan menggunakan uji One-way ANOVA menunjukkan terdapat perbedaan yangsignifikan antara kelima kelompok perlakuan dengan nilai p = 0.000 (p < 0.05).Post Hoc Test menunjukkan perbedaan yang signifikan antara KP1 – KP2 dengannilai p = 0.011 (p < 0.05) serta perbedaan yang tidak signifikan antara KK – KP1dengan nilai p = 0.129, KP1 – KP3 dengan nilai p = 0.081, dan KP1 – KP4 dengannilai p = 0.052 (p > 0.05).Simpulan: Pemberian ekstrak buncis (Phaseolus vulgaris L.) tidak terbuktimenurunkan kadar kolesterol LDL tikus putih (Rattus norvegicus) modelhiperkolesterolemia.Kata kunci: Ekstrak buncis, LDL, Rattus norvegicus, hiperkolesterolemia.ABSTRACTBackground: Low Density Lipoprotein (LDL) cholesterol related with CoronaryHeart Disease (CHD) which is the most common death cause in the world. Beanscontains flavonoid, phytosterol, saponin, niasin, vitamin C, and fiber which havehypolipidemic effect. This study aimed to determine the effect of beans extract tothe LDL cholesterol level in white rats hypercholesterolemia model.Methods: This study was a laboratory experimental research using the posttest onlycontrol group design, that have done in Histology Laboratorium of Sebelas MaretUniversity Surakarta. Samples of research were 30 male white rats, Wistar strain.Control Group (KK) was given pellets and aquades for 28 days. Group 1 until 4(KP1-4) were given duck egg yolk 5 gram/200 gram BW white rat/day and PTU0,01% for 28 days. On days 15-28, Group 2 (KP2) was given simvastatin 0,72mg/200 gram BW white rat/day, Group 3 was (KP3) given beans extract 100 mg/200gram BW white rat/day, and Group 4 (KP4) was given beans extract 150 mg/200gram BW white rat/day. The datas of LDL cholesterol level were analyzed withOne-way ANOVA test and Post Hoc test.Results: Result of this study shows that LDL cholesterol levels of KK = 33.83 ±14.442 mg/dl, KP1 = 54.00 ± 27.943 mg/dl, KP2 = 18.83 ± 7.672 mg/dl, KP3 = 77.33± 20.954 mg/dl and KP4 = 80.17 ± 31.923 mg/dl. The statistical analysis with OnewayANOVA shows a significant difference in LDL cholesterol level among thefive groups of treatment with p = 0.000 (p < 0.05). Post Hoc Test shows a significantdifference in LDL cholesterol level between KP1 – KP2 with p = 0.011 (p < 0.05)and non significant difference in LDL cholesterol level between KK – KP1 with p= 0.129, KP1 – KP3 with p = 0.081, and KP1 – KP4 with p = 0.052 (p > 0.05).Conclusion: The beans extract (Phaseolus vulgaris L.) does not give any effect tothe decrease of LDL cholesterol level in white rats (Rattus norvegicus)hypercholesterolemia model.Keywords: Beans extract, LDL, Rattus norvegicus, hypercholesterolemia.