ABSTRAK
Latar Belakang : Biji Pepaya (Carica papaya L.) mengandung flavonoid yang dapat berefek diuresis. Penelitian ini bertujuan untuk mengetahui efek diuresis ekstrak biji Pepaya pada tikus putih jantan (Rattus norvegicus).
Metode Penelitian: Penelitian ini merupakan penelitian eksperimental laboratorium dengan the post test control group design, dilakukan di Lembaga Penelitian dan Pengujian Terpadu (LPPT) Universitas Gadjah Mada Yogyakarta pada Oktober 2015. Subjek penelitian berupa 30 ekor tikus putih jantan, galur Wistar berumur 2-3 bulan dengan berat ± 200 gram. Teknik sampling yang digunakan adalah purposive sampling. Subjek dibagi dalam 5 kelompok secara random, masing- masing kelompok terdiri dari 6 ekor tikus putih. Sebelum perlakuan tikus putih diadaptasikan selama 7 hari dan dipuasakan selama 48 jam. Kelompok I sebagai kontrol negatif diberi aquadest 3 ml dan kelompok II sebagai kontrol positif diberi larutan hidroklorotiazid 0,3 mg/ 3ml. Kelompok III diberi ekstrak biji Pepaya dosis I (244 mg/3 ml), kelompok IV diberi ekstrak biji Pepaya dosis II (366 mg/3 ml), dan kelompok V diberi ekstrak biji Pepaya dosis III (488 mg/3 ml). Pengukuran volume urin dilakukan setiap 4 jam selama 16 jam setelah perlakuan.
Hasil Penelitian: Hasil uji one way-Anova volume urin tikus pada 4 jam pertama menunjukkan nilai p < 0.05 hal ini menunjukkan adanya perbedaan yang nyata. Hasil uji Kruskal-Wallis pada 4 jam kedua, ketiga dan 4 jam keempat memiliki nilai p > 0,05. Hal ini berarti tidak terdapat perbedaan volume urin yang bermakna pada 4 jam kedua, ketiga dan keempat. Hasil uji Mann Whitney yang dilakukan pada dua kelompok data dikatakan memiliki perbedaan bermakna apabila nilai p < 0,05, hasil uji menunjukkan perbedaan volume urin yang bermakna antara Kelompok Kontrol negatif dengan Kelompok Kontrol positif pada satu jam pertama.  Serta kelompok negatif dengan uji dosis III.  
Simpulan Penelitian: Terdapat perbedaan efek diuresis berbagai dosis ekstrak biji Pepaya (Carica papaya L.) pada tikus putih jantan (Rattus norvegicus).
Kata Kunci: ekstrak Carica papaya L., efek diuresis, hidroklorotiazid
ABSTRACT
Background: Seeds of Papaya (Carica papaya L.) contain flavonoids that can affect diuresis. Therefore, this study is aimed to determine the diuresis effect caused by a various doses of Papaya's seeds extract (Carica papaya L.) which is experimented to the male rats (Rattus norvegicus).
Methods: This study is an experimental research laboratory with the post test control group design, performed at the Institute for Integrated Research and Testing (LPPT) Gadjah Mada University in October 2015. The subjects of the research are a group of 30 male rats, which age is around 2-3 months old and weigh about 200 grams. The sample was chosen by using purposive sampling technique. The subjects were divided randomly into 5 groups, each group consisting of 6 rats. Before treatment, the rats were given an environmental adaptation for 7 days and then fasted for 48 hours. Group I as a negative control group received 3ml distilled water, while Group II as a positive control group received 3ml hydrochlorothiazide. The other three groups were being the experimental groups which is received the papaya's seeds extract. Group III got the first dose of Papaya's seeds extract (244 mg /3 ml), Group IV got the second dose (366 mg /3 ml), and  Group V got the third dose (488 mg /3 ml). The data were collected by gathering the urine of the rats which volumes were measured every 4 hours for the 16 hours after the aforementioned treatment were given.
Results: The results of one way-Anova showed that there is a significat difference of the rats' urines volume from different groups when tested in the first 4 hours (p < 0.05). When it was continued with Kruskal-Wallis test, the result showed that at the second, third and fourth next 4 hours, there is no significant difference between the rats' urine volume in different groups (p > 0.05). The Mann Whitney test which is conducted among two groups of data  indicated that ther are significant difference of the rats' urine volume between Group I and Group II and also Group I and Group V, in the first 4 hour testing (p < 0.05).
Conclusions: : There are differences in male rats (Rattus norvegicus) diuresis caused by extraction of Papaya's seed (Carica papaya L.)
Keywords: extract of Carica papaya L., diuresis effect, hydrochlorothiazide