ABSTRAKLatar Belakang : Insidensi terjadinya Demam Berdarah Dengue di Indonesiatergolong tinggi. Cara pencegahan terbaik adalah dengan memutus rantaipenularannya, salah satunya dengan larvasida. Ekstrak metanol Daun Anting-Anting diketahui dapat membunuh larva Aedes aegypti di India. Di sini penelitiingin meneliti apakah ekstrak etanol Daun Anting-Anting yang berasal dariIndonesia juga memiliki efek larvasida terhadap Aedes aegypti karena Anting-Anting yang ada di Indonesia kemungkinan memiliki variasi yang berbeda.Metode Penelitian: Penelitian ini merupakan eksperimental laboratorik yangdilaksanakan di Laboratorium Balai Besar Penelitian dan Pengembangan Vektordan Reservoir Penyakit (B2P2VRP) Salatiga, Jawa Tengah. Subjek penelitianadalah Daun Anting-Anting yang diperoleh dari daerah Sleman, Yogyakartadengan indikator penelitian berupa larva Aedes aegypti instar III berjumlah 750ekor yang diperoleh dari B2P2VRP Salatiga, Jawa Tengah. Ekstrak etanol DaunAnting-Anting diperoleh dari Laboratorium Farmasi Universitas Gadjah Mada.Rancangan penelitian yang digunakan adalah the post test only control groupdesign dengan 1 kelompok kontrol dan 5 kelompok perlakuan. Setiap kelompokberisi 25 larva dan dilakukan 4 kali ulangan. Kelompok kontrol diberi aquadest100 ml dan kelompok perlakuan diberi ekstrak etanol Daun Anting-Anting dengankonsentrasi masing-masing 0,03%, 0,06%, 0,09%, 1,2% dan 1,5%. Setelah 24jam, dilakukan pengamatan dan dihitung jumlah larva yang mati. Data kemudiandianalisis dengan Regresi Probit dan Curve Estimation.Hasil Penelitian: Persentase kematian larva Aedes aegypti pada 0,03%, 0,06%,0,09%, 1,2% dan 1,5% ekstrak etanol Daun Anting-Anting masing-masing adalah58%, 63%, 71%, 91% dan 94%. Hasil analisis Regresi Probit menunjukkan bahwaLC50 dan LC99 ekstrak etanol Daun Anting-Anting (Acalypha indica Linn.)berada pada konsentrasi 0,029% dan 0,61%. Sementara itu hasil analisis CurveEstimation menunjukkan adanya efek ekstrak etanol Daun Anting-Anting dengandengan kuat hubungan R² = 0,888 dan persamaan regresi linear Y = 0,478 + 3,073X.Simpulan Penelitian: Ekstrak etanol Daun Anting-Anting memilik efek terhadapmortalitas larva Aedes aegypti dengan LC50 pada konsentrasi 0,029% dan LC99pada konsentrasi 0,61%.Kata kunci : larva Aedes aegypti, ekstrak etanol Daun Anting-Anting, LC50 andLC99ABSTRACTBackground : The incidency of Dengue Hemorrhagic Fever (DHF) in Indonesiais high. The best way of prevention is cutting the infection chains, one of them isusing larvasida. Methanol extract of cat’s nettle leaf is known as the killer ofAedes aegypti larvae in India. Here, the researcher wants to examine whetherethanol extract of cat’s nettle leaf in Indonesia has same larvasida affect towardAedes aegypti. It is because the cat’s nettle leaf in Indonesia may have differentvariations.Methods : This research was laboratoric experimental which was did in BalaiBesar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP)Salatiga, Central Java. The research subject was cat’s nettle leaf which wasobtained from Sleman, Yogyakarta. Also, the research indicator was 750 thirdinstar Aedes aegypti larvas which were obtained from B2P2VRP Salatiga, CentralJava. Ethanol extract of cat’s nettle leaf was obtained from PharmacologyLaboratory of Gadjah Mada University. The research design was post test onlycontrol group design with 1 control group and 5 treatment groups. Each groupcontained 25 larvae and was examined forth times. Controlled group was addedaquadest 100 ml and treatment groups were added ethanol extract of cat’s nettleleaf with each concentration 0,03%, 0,06%, 0,09%, 1,2% and 1,5%. After 24hours, observation and calculation of how much the larvae is dead wereestablished. The data was analyzed by using Probit Regression and CurveEstimation.Results : The percentage of dead larvae in 0,03%, 0,06%, 0,09%, 1,2% and 1,5%of cat’s nettle leaf’s ethanol extract were 58%, 63%, 71%, 91% dan 94%respectively. The statistic result of Probit Regression shows that LC50 and LC99are in 0,029% and 0,61% concentration respectively. Also, the Curve Estimationresult shows that there is effect of ethanol extract of cat’s nettle leaf to themortality of Aedes aegypti larvae with tight correlation R² = 0,888 and the linearregression equation is Y = 0,478 + 3,073 X.Conclusion: The ethanol extract of cat’s nettle leaf has effect to the mortality ofAedes aegypti larvae with LC50 in 0,029% concentration and LC99 in 0,61%concentration.Key words: Aedes aegypti larvae, ethanol extract of cat’s nettle leaf, LC50 andLC99