Background: Peritoneal adhesion is considered as a patologic healing proccess after peritoneal injury, particularly because of surgical operation. Well-balanced between deposition and fibrin degradation is really important in determining normal peritoneal healing proccess or adhesion formation. Peritoneal adhesion has a significant economical effect. In US, approximately 117 inpatient cases associated with adhesion per 100.000 persons. Post surgical adhesion had a profound impact, including surgical procedure itself, inpatient cases, healing proccess, and loss of productivity.
Aim : Knowing the difference effect between normal saline and water sterile for irrigation as a washing liquid in peritoneal adhesion formation in trial animals, wistar mice.
Methode : This research is using post only control group methode, with laboratory experimental design. Research sample consisted of 32 wistar strain mouse age 9-12 weeks, weighted 200 gr, 16 mice from them will be treated with normal saline and the other 16 mice were given sterile water. Adhesion stage was measured from macroscopic form, based on Zuhike methode. The data will be analyzed using SPSS 17.0, α = 0.015 with non parametric statistical test. Comparing the difference effect between intervention group by using Chi Square test.
Results : 16 trial samples treated with NaCl 0,9% has an adhesion grade 1, grade 2, and grade 3, with total number of 6 mice. While most of the sample did not have an adhesion, in total 10 experimental animals. While the 16 mice given sterile water, appeared to have an adhesion with grade 0 or did not have adhesion. There is a tendency that the administration of NaCl 0.9% would increase the occurrence rate of adhesion between the intestine. With P value = 0.061, means there was no significant difference in the adhesion degree between the group given 0.9% NaCl as well as in the group given sterile water. 16 samples of mice were given 0.9% NaCl are all having adhesion, with 14 mice are having second degree of adhesion and 2 subjects are having fourth degree of adhesion. While the 16 mice given sterile water, 6 subjects are having grade 0 of adhesion or did not occur at all, while the remaining 10 samples, 9 mice are having a second grade of adhesion and one sample is having grade 3 of adhesion. So there is a likelihood that administration of NaCl 0.9% would increase the occurrence rate of adhesion in stitches.
P value = 0.018, (p <0.05), which means there was a significant difference of the adhesion grade between the group given 0.9% NaCl and the group given sterile water, but the group given NaCl 0.9% tend to have a higher degree of adhesion than the group treated by using sterile water.
Conclusion: There is no significant difference between the administration of normal saline with sterile water as washing liquid on adhesion formation between the intestine in wistar mice. There is a significant difference between the administration of normal saline with sterile water as washing liquid into peritoneal adhesion formation in the area of stitches in Wistar mice
Keywords: Adhesion, normal saline, sterile water