Backgroumd : Lupus nephritis is a common and serious complication in SLE with deposition of autoanti-bodies in the glomerulus. HsCRP is an acute-phase reactant serum which usually parallel with disease activity in inflammatory states. TNF ? was reported to be increased in Lupus Nephritis and correlated with disease activity and it has been proposed to contribute to the immunopathogenesis of SLE. Injection Pristan intraperitoneal can induce lupus Nephritis in mice. Secretome mesenchymal stem cells act as anti-apoptotic agents, suppress B cells and autoreactive T cells, and have anti-inflammatory properties

Aim : This study aimed to determine the effect of secretome mesenchymal stem cells on the HsCRP levels and expression of TNF ? in mice model of Lupus Nephritis.

Methods : An experimental study with randomization, with post test only control group design, the sample of 21 female mice Mus musculus strain Balb / C, divided into 3 groups: control group (intraperitoneal injection of 0.5 ml of 0.9% NaCl), the group Pristan (injection Pristan intraperitoneal 0.5 ml) and Pristan + secretome group (intraperitoneal injection of 0.5 ml Pristan and secretome 0.45 ml). Research carried out for 24 days, secretome given on the day 21st of the study. After treatment, we performed measurements of HsCRP levels and TNF expression. Statistical analysis using SPSS 22 for windows with ANOVA and post hoc LSD. p significant if p <0.05.

Result : There are statistically significant differences in all groups in HsCRP levels (p=0,003 ) and TNF ? expressions (p=0,005). There is a difference in hsCRP levels between the control (387.05 pg/mL +157pg/mL) with Pristan (2152.92pg/mL+1440.93pg/mL) (p=0,002), Pristan with Pristan+sekcetome (686.33pg/mL+524.91pg/mL) (p=0,006), and there is no difference between the control group with Pristan+secretome (p=0,537). There is a difference in TNF ? expressions between the control (6.86/100 limfosit+1.95/100 limfosit) with Pristan, (11.71/100 limfosit+ 3.40/100 limfosit) (p=0,002), Pristan with Pristan+sekcetome (7.86/100 limfosit+2.04/100 limfosit) (p=0,011), and there is no difference between the control group with Pristan+secretome (p=0,473).

Conclusion : Secretome mesenchymal stem cells can decrease HsCRP levels and expression of TNF ? in mice model of Lupus Nephritis.

                                                                                                                                       Keyword : Secretome mesenchymal stem cells, HsCRP, TNF ?, Lupus Nephritis