Nile tilapia (Oreochromis niloticus) is currently one of the main species of aquaculture, making it important to world fish farming. Supplementation of enzymes in feed is one of the possible options for improvement of the nutritional value of fish feed. This study was conducted to know the effect of phyprozyme HP supplemented diets on growth performance and body weight composition of Nile tilapia. Phyprozyme HP enzymes are standardized to contain a combination of Protease, Xylanase, Hemicellulose, and Phytase. The Mean Final Weight (MFW), Weight Gain (WG), Average Daily gain (ADG), Feed Conversion Ratio (FCR), Specific Growth Rate (SGR), and Survival Rate (SR) were determined. This research used Completely Randomized Design (CRD) with eight treatments and two replicates: T1 control (0.0), T2 (0.05), T3 (0.12), T4 (0.19), T5 (0.26), T6 (0.33), T7 (0.40), and T8 (0.47) g phyprozyme HP/ kg-1 feed. Nile tilapia with the average initial weight of (11.9 ± 8.6 g) were stocked into hapa nets (70×45×90 cm3) 20 fish / hapa net and were fed three times daily (8:00 AM, 12:00, and 16:00 PM) at feeding rate of 5% of the total body weight. After 56 days of feeding, there were significant differences (P<0>0.05). Proximate analysis of the carcass of O. niloticus indicated, there were no significant differences between the treatments in terms of moisture and protein contents of body composition. The highest value (81.52 ± 2.96) of moisture was observed with fish fed (T8) and the lowest value (77.65 ± 3.32) was obtained with fish fed (control). The highest value of protein (11.68 ± 0.72) was observed with (initial weight) and the lowest value (7.43 ± 0.12) was obtained with fish fed (control). There was a significant difference (P<0>

Key words: nile tilapia, phyprozyme hp, growth performance, body weight composition