Introduction: Various techniques for tissue engineering have been introduced to help regenerate damaged or lost bone tissue. The purpose of this study was to see the potential for platelet-rich fibrin (PRF) to accelerate the bone healing process in rat bone defects. Methods: A systematic literature search was conducted from several electronic databases on subjects looking at the use of PRF in rat bone defects and their results in bone regeneration. Specific results were comparisons of PRF vs other methods, PRF vs control and PRV vs combination PRF and other methods. Results: There were a total of 483 rats from 12 studies. This meta-analysis showed that the PRF vs other methods pooled odds ratio (OR) obtained was 0.92 (95% CI 0.42 – 2.04; p=0.29; I2=18%), PRF versus control OR obtained 9.45 (95% CI 4.68 – 19.08; P<0 I2=0%), p=0.0008; I2=0%).>