In teaching and learning reading, teachers have been using various teaching strategies, teaching methods, and learning approaches, but perhaps they do not know which teaching strategy is suitable for students’ learning. To know which teaching strategy is suitable for students’ learning, teachers need to know the students' preferences in learning or students’ learning styles. Therefore, this research was aimed to find whether there is a difference between learning styles: auditory, visual, and kinesthetic on students’ reading comprehension. The data on students’ learning styles were collected through documents from the school that had already been collected and analyzed by the Psychology Services Bureau in Indonesia. The data on students’ reading comprehension were collected through a reading test based on reading competency standards by Depdiknas (2007). The researcher applied an ex post facto method and used a one way analysis of variance (ANOVA) to find the difference between auditory, visual, and kinesthetic learning styles on students’ reading comprehension. The finding showed that there is a significant difference between learning styles and reading comprehension with P Value 0.001. Knowing that learning styles have significant differences on reading comprehension, this study suggests teachers and education providers be aware of learning styles and include analysis of students' learning styles in designing activities of teaching and learning reading that may be beneficial to help students develop reading comprehension.