Chemical fertilizers could accelerated and maximized cayenne pepper yields, but continuous use of anorganic fertilizers could reduced organic matter content, soil microorganism activity and environmental pollution. Types of environmentally friendly fertilizers such as biological fertilizers and vermicompost are recommended as substitutes for chemical fertilizers. This research aimed to studied the effect of effective doses of biological fertilizers and vermicompost on the growth and yield of cayenne pepper. The research was carried out in April-September 2021 at the screen housed of Building C, Faculty of Agriculture, Universitas Sebelas Maret. The method used was a factorial Randomized Completely Block Design (RCBD) with two factors, namely the dose of biofertilizer (0 L/ha, 15 L/ha, and 20 L/ha). The second factor is the ratio of soil (control, 4:1, 4:2, and 4:3). The study used 3 replications. Parameters observed were plant height, number of leaves, stem diameter, fresh weight of roots and shoots, dry weight of roots and shoots, cumulative number of fruit per plant, fruit length, cumulative fruit weight per plant. Data used Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT)  at 5% level. The results showed that the single treatment of liquid biological fertilizer had no significant effect on all growth parameters and yields of cayenne pepper. Single treatment with a ratio of soil and vermicompost (4:1) was able to increase the number of cayenne pepper leaves. The combination of 15L/ha liquid biological fertilizer treatment and the ratio of soil to vermicompost (4:3) was effective in increasing the wet and dry weight of plants.